Figure 2. Both TAK1^KO and TAK1^WT cells treated with takinib + TNF are phenotypically distinguishable from TAK1^WT.

(A) Different levels of pro- and anti-apoptotic markers are found in MDA-MB-231 TAK1^WT vs TAK^WT treated with takinib + TNF. (B) TAK1^WT MDA-MB-231 cells were treated with either vehicle, TNF (30 ng/mL), or takinib (10 μM) + TNF for 12 hours. Compared to untreated TAK1^WT cells, takinib + TNF combination therapy saw an upregulation in apoptotic markers. Cleaved caspase-3, cytochrome c, HTRA2 and Livin levels were higher in takinib + TNF treated cells, compared to both vehicle and TNF only treatments. n = 3 ± SEM, Two-way ANOVA.