Figure 3. The efficacy of TAK1 inhibition on cell death is characterized by TAK1^KO, as well as takinib + TNF treatment.

(A) An apoptosis protein array revealed that in comparison to TAK1^WT (with Cas9 vehicle), TAK1^KO upregulated several apoptotic proteins. (B) Despite some similarities in apoptotic biomarkers, TAK1^KO were significantly more sensitive to apoptosis following TNF (30 ng/mL) treatments. (C) Apoptotic protein expression in TAK1mediated TNF apoptosis. (D) Differences in protein levels were evident in TAK1^KO vs. TAK1^KO with TNF proteome assays. (E) Compared to TNF and takinib + TNF treatments, TAK1^KO cells that were treated with vehicle, had significant upregulation of Hsp60, Cleaved Caspase-3 and SMAC. Two-way ANOVA.