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. 2020 May 29;3:273. doi: 10.1038/s42003-020-0979-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Top left: cartoon depicting the strategy for reporter gene fragmentation, applied for each RP/MP gene included in the Tol ORF. Top right: reporter proteins included in the Tol ORF. Bottom: schematic overview of gene fragment placement in the Tol ORF. Note that the Tol ORF encodes the HPV E7_49–57 epitope in the COOH-terminal region of the chimeric Tol protein. b Cartoon depicting the targeting strategy of the Tol ORF into the Col1a1 locus through recombinase-mediated cassette exchange. c Differential gene expression analysis of indicated organs of Tol mice relative to WT mice. Scatterplots indicate average log2 fold changes and average counts per million. Genes with a statistically significant change in expression in Tol mice are indicated in gray, and the Tol transcript is indicated in blue. d Expression levels of the Tol transcript in thymi of Tol and WT mice. Data shown (c, d) is aggregated from n = 4 (Tol) and n = 4 (WT) mice. RP reporter protein, MP modifier protein, RMCE recombinase-mediated cassette exchange, PURO-R puromycin resistance gene, TK thymidine kinase gene, CPM counts per million.