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. 2020 May 29;10:8754. doi: 10.1038/s41598-020-65602-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Survival decreased sharply with exposure to increasing sub-inhibitory ciprofloxacin concentrations. Percent survival (relative to no drug treatment) plotted against ciprofloxacin (Cip) concentration (% MIC noted below) after (a) 24 hr exposure and (b) 48 hr exposure. Error bars represent the standard deviation of the mean of 3 independent experiments. For better resolution of small values, inset shows survival at ≥20% MIC Cip exposure, when survival is less than 20%. The growth observed at 100% and 110% (~50–100 CFU/ml) at 24 hrs was from 1 experiment. Inset is on a log scale, and thus values of 0 are not plotted.