Fig. 6. Mitochondrial axonal transport is disrupted by TRPV4^R269C prior to axonal degeneration.

a Kymographs of mito-GFP transport in the proximal axon of C4da neurons expressing the indicated TRPV4(mod) variants. Scale bar, 10 μm. Quantification of b anterograde duty cycle and c retrograde duty cycle. For control, TRPV4^WT, TRPV4^R269C, TRPV4^R269C+M680K n = 10, 10, 9, 8 and 7, 11, 7, 5 larvae for anterograde and retrograde, respectively. Mean ± SEM. Two-way ANOVA (p < 0.0001), Tukey’s post hoc test. Frequency distributions of d anterograde and e retrograde mitochondrial run lengths. For control, TRPV4^WT, TRPV4^R269C, TRPV4^R269C+M680K: n = 12, 11^, 14, and 10 larvae; 138, 108, 77, and 72 anterograde and 63, 86, 30, and 29 retrograde runs, respectively. For all panels: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.