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. 2020 May 29;3:274. doi: 10.1038/s42003-020-0997-y

Fig. 4. Focused refinement of ACP stalled at the DH domain.

Fig. 4

a Focused region and signal subtracted region are shown in gray and transparent light gray, respectively. Two slices of the unsharpened map are shown to the left. b Atomic models (PDB: 2UV8) flexibly fitted to sharpened map. Distances between catalytic residues5 of DH (Cδ of D1559 and Nɛ of H1564) and ER (Nɛ of H740) domains to the Cβ of S180 of ACP are given in parenthesis. c Focused 3D classification showing domain mobility of ACP stalled at DH. Particle distribution was ~50% for both 3D classes. Both maps are shown at the same threshold and low-pass filtered to 10 Å.