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. 2020 May 29;10:8802. doi: 10.1038/s41598-020-65782-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of kefir peptides on migration of THP-1 monocytes. (A) Flow chart of the experimental setting. (B) Representative fluorescent images showing CM-DiI-labeled THP-1 monocytes which migrated across the transwell insert membrane to the lower site after 2 h incubation in oxLDL CM or Ctrl CM with or without kefir peptides (KPs, 100 μg/ml). (C) The histogram shows the quantification data of three independent migrated cells on the lower site of transwell insert per high power field determined by ImageJ. Scale bar: 200 μm. (D) The histogram shows the number of three independent migrated cells pass through the transwell insert which suspend in the lower chamber were counted using hemocytometer. The statistical analysis was performed according to Duncan’s multiple-range method. The labels at the top of columns without the same letters indicate significant differences between groups (P < 0.05).