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. 2020 May 29;11(5):406. doi: 10.1038/s41419-020-2618-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a PLC/PRF/5 (n = 6) and Huh7 cells (n = 4) were treated with either vehicle or 5 ng/mL of TGF-β1 for 2 days. At 1 h before the measurement, tBHP was added at doses of 100 µM to PLC/PRF/5 cells (n = 3) and 50 µM to Huh7 cells (n = 4)(right). b Cells (n = 3) were pretreated with 20 µM Fer-1 1 h before TGF-β1 treatment. DFOA was added at doses of 100 µM 1 h before the measurement in PLC/PRF/5 cells or before tBHP treatment in Huh7 cells. TGF-β1 and tBHP were treated as described in a. The first two groups in Huh7 cells (tBHP, TGF-β1 with tBHP) are identical to a as these experiments were performed at once, but displayed again to enhance understanding. c PLC/PRF/5 (n = 4) and Huh7 cells (n = 4) were treated with either vehicle or 5 ng/mL TGF-β1 for 8 days. tBHP was added 1 h before measurement at doses of 100 µM in PLC/PRF/5 cells (n = 4) and 50 µM in Huh7 cells (n = 4). d SK-Hep1 (n = 3) and SNU475 (n = 3) cells were treated with 5 ng/mL of TGF-β1 for 2 days and then further incubated with tBHP at the indicated concentrations for 1 h. Lipid peroxidation was measured with the BODIPY® C11 probe and expressed as the mean fluorescence intensity (MFI); multiple analyses are shown as the mean ± SD. Representative histograms were shown in supplemental Fig. 1. *P < 0.05, **P < 0.01, TGF-β1 versus vehicle; †P < 0.05, ††P < 0.01, TGF-β1 in vehicle-treated cells versus TGF-Β1 in Fer-1- or DFOA-treated cells.