Fig. 2.

Ability of CK1 inhibitors to block TDP-43 aggregation. NSC-34 cells were treated with 0.1 µM tunicamycin (Tn) ± 10 µM D4476 or 10 µM PF670462, as indicated. Cells were subsequently fixed and a immunocytochemistry was performed as described, using primary antibodies against TDP-43 using a 20 × objective. Scale bars, 50 µm and b the percentage of cells with cytoplasmic inclusions was calculated. Each data point refers to one field of view, where n = 4 (biological replicates). Statistical significance was assessed using one way ANOVA with Tukey’s post hoc test. Horizontal lines (blue) indicate mean and SD