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Different grades of p16 immunohistochemistry based on the percentage of immunopositive cells. a (200×) Grade 1: 0 to 25% immunopositive cells. b (200×) Grade 2: 25 to 50% immunopositive cells. c (200×) Grade 3: 50 to 75% immunopositive cells. d (200×) Grade 4: 75 to 100% immunopositive cells. For the purpose of analysis only, grade 4 cases with intensity of 2/3+ were considered positive for p16
