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Chondrocyte-specific protein expression of hNCs in 2D monolayer culture. A Confocal images of cultured hNCs and hACs double stained with the chondrocyte-specific markers Sox9 (green), type II collagen (red), and DAPI (blue). Scale bars: 50 μm. B Western blots of SDS-PAGE gels of hNCs extracts obtained from five different donors and immunodetection using antibodies against Sox9 and type II collagen. β-actin was used as a loading control
