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. 2020 May 29;10:8777. doi: 10.1038/s41598-020-65324-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Representative platelet poor plasma (PPP) smears with added anti-E. coli LPS antibody and secondary antibody, from healthy individuals and colorectal cancer (CRC) patients. Micrographs were enhanced for publication clarity, by adjusting the brightness and contrast of the images in Adobe Photoshop CS6. (B) Box and whisker plot of the normalised (to secondary antibody control signal) mean fluorescence intensities (MFIs) of healthy (n = 29) (left box) and CRC (n = 25) (right box) PPP smears, with added anti-E. coli LPS antibody and secondary antibody.