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. 2020 May 29;11(5):405. doi: 10.1038/s41419-020-2627-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Relative IL-8 mRNA expression was detected using PCR in HNSCC patients, including primary and recurrent tumors (n = 106), and healthy controls (n = 111). b Relative IL-8 mRNA expression was detected in HNSCC cell lines, normal oral mucosa, and HIOEC. c Expression of IL-8 by HN4, HN6, Cal27, HIOEC, and normal oral mucosa detected by ELISA. d Survival analysis was performed in the HNSCC dataset from the TCGA database. The correlation between IL-8 expression and tumor recurrence (e), lymph node metastasis (f), TNM stage (g), age (h), smoking (i), alcohol use (j), and gender (k) was analyzed in HNSCC patients. Representative IHC images of IL-8 correlation with tumor status (l), advanced stage (m), and lymph node metastasis (n) are shown (magnification 400×). Scale bar, 50 μm; *P < 0.05, **P < 0.01.