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. 2020 May 29;11(5):405. doi: 10.1038/s41419-020-2627-5

Fig. 2. IL-8 promotes malignant progression in HNSCC cell lines.

Fig. 2

a Migration ability of HNSCC cells treated with the indicated IL-8 concentration was detected by a wound healing assay. Scale bar, 100 μm. b, c Transwell assays were performed to determine the migration and invasion ability of cells with different concentrations of IL-8. Scale bar, 250 μm. d Colony formation ability of cells in three groups was determined using a colony-forming assay. e The CCK8 assay was used to detect the proliferation ability of HNSCC cells with different concentrations of IL-8. f E-cadherin, MMP2, MMP9, and vimentin were detected using western blot after 100 ng/ml IL-8 stimulation; *P < 0.05, **P < 0.01.