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. 2020 May 29;11(5):405. doi: 10.1038/s41419-020-2627-5

Fig. 6. The inactivation of PTEN plays a pivotal role in STAT3 activation and IL-8 secretion.

Fig. 6

a The expression levels of PTEN, p-PTEN, STAT3, and p-STAT3 were examined after 100 ng/ml IL-8 and/or PTEN stimulation. b The fluorescent images of Cal27 cells treated with rhPTEN-FITC or FITC alone for 24 h. Scale bar, 200 μm. c Cal27 cells incubated with rhPTEN-FITC and DY-554 phalloidin were detected by confocal immunofluorescent analysis. White arrows indicate the rhPTEN-FITC internalized by HNSCC cells. Scale bar, 50 μm. d PTEN, STAT3 and p-STAT3 expression was detected by western blot after transfection with si-PTEN or si-scramble. e–g The transfection efficiency was validated by western blot. h ELISA was performed to detect the secretion of IL-8 in HNSCC cells after transfection with si-PTEN and/or snail vector; **P < 0.01.