Figure 1.

Protein expression profile of MECPK cells, normal uterine tissue, and Pten^−/−K-ras^G12D uterine tumor. Western blot analysis of PTEN, Phospho-AKT (pAKT), AKT, PGR, and ESR1 in MECPK cell line extract as compared to normal uterine tissue and uterine tumor tissue from Pten^−/−K-ras^G12D mice. i) MECPK cells completely lack PTEN as compared to normal tissue and tumor tissue samples indicating purity of the cell line and lack of stromal contamination as seen in the faint banding of the tumor samples. ii) MECPK and mouse uterine cancers have elevated levels of pAKT as compared to normal uterine tissue while total AKT (iii) between the samples remained relatively constant. iv-v) Both estrogen and progesterone receptors (ESR1 and PGR) are undetectable in the MECPK cell line. vi) β-actin serves as the loading control. 10 μg protein/lane. Membranes were stripped and re-probed for each antibody. Full, non-adjusted images of blots are provided as Supplemental Figure 4.