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. 2020 May 27;6(5):e04075. doi: 10.1016/j.heliyon.2020.e04075

Figure 2.

Figure 2

MECPK model method, primary tumor formation, and immune gene expression. A) Summary of graft protocol. For three consecutive days prior to surgery, mice were injected with 0.1 μg of estrogen. The distal ends of the uterine horns were ligated and the ovaries removed (OVX) on the day of surgery. 500,000 cells were injected into one horn suspended in a 1:1 mixture of PBS:Matrigel. The second horn was left as a control and received a sham surgery. B) Representative tumor formation in a mixed background immune-competent mouse (i) with corresponding detection of GFP labeled cells (ii). GFP labeled cancer cells invade into uterine wall (iii-iv). C) Immune gene expression in normal uterine (control) n = 3 and tumor bearing uterine tissue (tumor) n = 3 (left). Selected immune gene expression in MECPK-GFP cells (right). Immune gene expression was normalized to Ppia, a housekeeping gene.