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. 2020 May 30;31(11):107774. doi: 10.1016/j.celrep.2020.107774

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© 2020 The Author(s)

Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

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In Vitro Polymerase Activity of nsp12 and Regulatory Effects of Cofactors

(A) Comparison of RNA synthesis activities of the SARS-CoV-2 and SARS-CoV core polymerase complex. Efficient activity of nsp12 polymerase requires the presence of the nsp7 and nsp8 cofactors. Apart from the fully elongated product (green arrowhead), some aberrant termination products were also observed (yellow arrowhead). The excess primer band is indicated by a cyan arrowhead.

(B and C) Comparison of the regulatory effects of the nsp7 and nsp8 cofactors in the context of the SARS-CoV-2 (B) and SARS-CoV (C) nsp12 polymerase, respectively.

(D) Comparison of the activity of the nsp12 polymerase of different viruses in the same context of cofactors. Polymerase activity was quantified by integrating the intensity of the fully elongated product bands. The results, in histograms, are presented as the means ± SD, where error bars represent SDs. The significance of difference was tested by one-way ANOVA based on the results of four independent experiments (n = 4) using different protein preparations. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, ^∗∗∗∗p < 0.0001.

See also Figure S4.