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. 2020 May 25;14:2021–2031. doi: 10.2147/DDDT.S248104

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© 2020 Chen et al.

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Down-regulation of miR-19a-3p caused by glycine inhibited cell apoptosis, glucose metabolism disorder and inflammatory response.

Notes: (A) Down-regulation of miR-19a-3p enhanced cell activity when * was 96 h, P<0.05. (B) Down-regulation of miR-19a-3p reduced TNF-α, IL-1β and IL-6. (C) Down-regulation of miR-19a-3p up-regulated Glut1, HK2, LDHA, PDK1, PKM2. (D) Down-regulation of miR-19a-3p inhibited Caspase 3, Caspase 9, Bax and promoted Bcl2. (E) Western blot band of each protein. (F) Down-regulation of miR-19a-3p reduced the apoptosis rate. (G) Down-regulation of miR-19a-3p up-regulated AMPK, p-AMPK, GSK-3β, p- GSK-3β, Nrf2, Ho-1. *P<0.05, **P<0.01. The experiments were repeated for three times.

Abbreviations: OD, optical density; Glut1, Glucose transporter type 1; PKM2, Pyruvate Kinase M2; HK2, Hexokinase 2; LDHA, Lactate Dehydrogenase A; PDK1/2, Pyruvate Dehydrogenase Kinase 1/2; TNF-α, Tumor Necrosis Factor α; IL-1β, interleukin-1β; IL-6, interleukin-6; Bax, BCL2 Associated X, Apoptosis Regulator; Bcl2, BCL2 Apoptosis Regulator; PI-A, propidiumiodide; Annexin V-FIFC, Fluorescein isothiocyanate; AMPK, Adenosine 5‘-monophosphate (AMP)-activated protein kinase; p-AMPK, AMPK phosphorylation; GSK-3β, Glycogen Synthase Kinase 3β; p-GSK-3β, GSK-3β, phosphorylation; HO-1, Heme Oxygenase 1; Nrf2, Nuclear Factor Erythroid 2-Related Factor 2; OGD, glycose-oxygen deprivation; NC, negative control.