Figure 1.
Melatonin ameliorated A/R injury in H9c2 cells by increasing cellular viability and reducing the apoptotic index, cellular Ca2+ level, LDH release and CK-MB level, but these effects were attenuated by 4-P-PDOT or 3-TYP and increased by IIK7. (A–D) Cell viability was examined by MTT assay and was calculated by dividing the optical density of samples by that of control group. (E) Apoptotic cells were evaluated by Annexin V/PI staining; the results are expressed as the calculated apoptotic index. (F) The mean fluorescence of Fluo-3 AM-stained cells was assessed using a confocal microscope, and the data were normalized to the control group. (G) LDH levels. (H) CK-MB levels. (I) Representative apoptosis data from flow cytometry. (J) The fluorescence intensity of Fluo-3 AM is representative of the cellular calcium concentration. Data are described as the mean ± SEM (n=6 in each group). *P < 0.05 vs the control group; #P < 0.05 vs the A/R group; &P < 0.05 vs the A/R + Mel group.