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. 2020 May 16;34:101570. doi: 10.1016/j.redox.2020.101570

Fig. 5.

Fig. 5

Expression of genes in pancreatic islets at different oxygen levels and in the presence of cytokines. Target gene mRNA expression of Bax, Bcl2, c-FLIP, HIF1α and VEGF-A in porcine pancreatic islets cultured for 24 h under normoxia (20% oxygen) or hypoxia (2% oxygen) and in the presence of IFN-γ (100 ng/ml), IL-1β (5 ng/ml), and TNF-α (10 ng/ml) and 30 or 60 μg/ml hTrx1 or as control 60 μg/ml denatured hTrx1 (dhTrx1) or redox inactive hTrx1 C32S (C32S), respectively. The ratio of the mRNA expression of the proapoptotic gene Bax and antiapoptotic gene Bcl2 is given instead of the respective sole expression. Upon hypoxia, treatment with 60 μg/ml hTrx1 significantly reduced the relative mRNA expression ratio of Bax/Bcl-2 by 46%. Upon cytokine treatment during normoxia and hypoxia, as well as upon hypoxia without cytokine treatment, HIF-1α expression increased 2.9-fold, 2.6-fold and 2.4-fold, respectively. 60 μg/ml denatured dhTrx1 and the redox-inactive C32S served as controls and did not show any significant effects. Treatment time was 24 h ****p < 0.0001, ***p < 0.001, **p < 0.005, *p < 0.05 (one-way ANOVA), n = 9.