Figure 2.

Tumorigenesis Test In Vivo of Liver Cancer Cells

(A) Comparison of the tumor formation rate of liver cancer cells in nude mice. (B) In (a), Hep3B cells stably infected with rLV and rLV-miR-155 lentivirus were inoculated into the skin of BALB/c nude mice. (b) Photographs of transplanted tumors (xenografts) dissected from of BALB/c nude mice. (C) Comparison of the size (in grams) of xenografts. (D) Histopathological analysis of transplanted tumors formed in nude mice. A 4% formaldehyde-fixed, paraffin-embedded nude mouse transplanted tumor tissue section (4 μm) was subjected to hematoxylin and eosin (H&E) (original magnification, ×100). (E) In (a), immunohistochemical analysis of transplanted tumor tissue formed in nude mice. Immunohistochemical staining of anti-PCNA and anti-Ki67 (original magnification, ×100) was performed. (b) The comparison of PCNA-positive rates of xenograft. (c) The comparison of Ki67-positive rates in transplanted tumors in nude mice.