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. 2020 May 8;17:471–483. doi: 10.1016/j.omto.2020.05.002

Figure 3.

Figure 3

Analysis of miR-155 Targeting H3F3A

(A) RNA sequencing analysis. (B) The analysis of mature miR-155 seed sequence binding to the H3F3A mRNA 3′ UTR. (C) pMirtarget-H3F3A 3′ UTR-Luc luciferase reporter gene activity was detected. (D) RT-PCR was used to detect H3F3A . β-actin was used as an internal reference gene. (E) Western blotting was used to detect the expression of H3.3. β-actin was used as an internal reference gene.