Table 1.
Summary of HiTrap Heparin Column Binding Studies
| AAV Capsid | AAV Predominantly Detected in: | [NaCl] at Elution Peak Maxima (mM) |
|---|---|---|
| AAV2 | elution fraction | 453 |
| AAV2.V5 (AAV2 T503A+N596D) | elution fraction | 371 |
| AAV2.V12 (AAV2.V59 A503T+D596N) | elution fraction | 463 |
| AAV2.V59 | elution fraction | 368 |
| AAV2-N496D | elution fraction | 200 |
| AAV2-N582S | elution fraction | 362 |
| AAV2-K532E | elution fraction | 282 |
| AAV2-6M | flowthrough | – |
| AAV8 | flowthrough | – |
| AAV8-RQNR | elution fraction | 650 |
| AAV8-E533K | elution fraction | 369 |
| AAV8-RQNR-N499D | elution fraction | 460 |
| AAV8-E533K-N499D | elution fraction | 271 |