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. Author manuscript; available in PMC: 2020 Oct 7.
Published in final edited form as: Mol Pharm. 2019 Aug 26;16(10):4302–4312. doi: 10.1021/acs.molpharmaceut.9b00639

Figure 7.

Figure 7.

Assessment of the anti-inflammatory response of the secretome146a. (A, B) qPCR analysis of main genes involved in the inflammatory response of HUVECs upon exposure to IL-1β. Gene profiles of ICAM, MCP-1, TRAF6, NOS3, and VCAM were studied at 4 and 24 h. (C) Quantification of MCP-1 secreted by HUVECs after being exposed to IL-1β and subsequent treatment with the different types of the secretome. (D) FACs analysis reporting the mean fluorescent intensity of positive fluorescent stained HUVECs expressing the cellular molecular adhesion protein ICAM. Results are reported as mean ± deviation standard. *p < 0.05, **p < 0.01 ***p < 0.001.