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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2019 Dec 26;198:105572. doi: 10.1016/j.jsbmb.2019.105572

Figure 1. Steroids adsorb to different vessels in a manner that depends on type of vessel and on steroid lipophilicity.

Figure 1.

(A) List of nine steroids used in these experiments along with experimentally determined log octanol-water partition coefficients (log Kows) obtained from Leszczynski and Schafer [9]. (B) Fraction of steroid retained after one hour incubation in D-PBS solution in four different types of microcentrifuge tubes as well as tissue culture plates and glass vials (see Materials and Methods for details of different vessels) for the nine steroids. All experiments were performed twice in duplicate; plots show each individual value with lines representing mean values. (C) Graphs of logs of fraction of steroid retained vs. log Kows for the nine steroids and six different vessels. Mean values ± standard deviations are shown. For all graphs, slopes of trendlines are different from zero (p < 0.0001).