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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2019 Dec 26;198:105572. doi: 10.1016/j.jsbmb.2019.105572

Figure 2. Chromatographic lipophilicity parameters confirm the relationship between steroid lipophilicity and adsorption.

Figure 2.

(A) List of nine steroids with retention times (tr) obtained using reverse phase high performance liquid chromatography (RP-HPLC; values are averages of two runs of all nine steroids), retention factors k = (tr − t0)/t0, and logs of retention factors. (B) Graphs of logs of fraction of steroid retained vs. log k for the nine steroids and six different vessels. Mean values ± standard deviations are shown. For all graphs, slopes of trendlines are different from zero (p < 0.0001).