Skip to main content
. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2019 Dec 26;198:105572. doi: 10.1016/j.jsbmb.2019.105572

Figure 3. Steroid adsorption increases with incubation time and with centrifugation.

Figure 3.

(A) Fraction of steroid retained in Fisher microcentrifuge tubes after incubation times of 5 minutes, 30 minutes, 2 hours, 7 hours, and 24 hours for two steroids, pregnenolone and DHEA. Experiment was performed twice in duplicate and points on graph show mean ± SD. (B) Fraction of steroid retained in Fisher microcentrifuge tubes after 1 min. 6000 g centrifugation. Experiment was performed twice in triplicate and graph shows mean ± SD. (C) Fraction of steroid retained in TC plates after the same incubation times as in (A). Experiment was performed twice in duplicate and points on graph show mean ± SD.