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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2019 Dec 26;198:105572. doi: 10.1016/j.jsbmb.2019.105572

Figure 4. Serum proteins prevent most of the steroid adsorption.

Figure 4.

Fraction of pregnenolone (A) or DHEA (B) retained in Fisher microcentrifuge tubes, and fraction of pregnenolone (C) or DHEA (D) retained in TC plates, after one hour incubation in distilled water, D-PBS, serum-free culture media, or culture media with 10% charcoal-stripped fetal bovine serum. Experiments were performed twice in duplicate; plots show each individual value with lines representing mean values. For pregnenolone in tubes, retention from 10% CSS media was less than from all other solutions and retention from serum-free media was less than from distilled water or D-PBS (p < 0.001 for all comparisons, Tukey’s multiple comparison test after one-way ANOVA). For DHEA in tubes, retention from 10% CSS media was less than from all other solutions (p < 0.001 for 10% CSS media vs. distilled water or D-PBS; p = 0.004 for 10% CSS media vs. serum-free media) and retention from serum-free media was less than from distilled water (p = 0.005) or D-PBS (p = 0.002). For pregnenolone in TC plates, retention from 10% CSS media was less than from all other solutions and retention from serum-free media was less than from distilled water or D-PBS (p < 0.001 for all comparisons). For DHEA in TC plates, retention from 10% CSS media was less than from all other solutions (p < 0.001 for all comparisons) and retention from serum-free media was less than from distilled water (p = 0.01) or D-PBS (p = 0.003).