Figure 5. Steroid concentration appears not to affect fraction adsorbed.
Fraction of pregnenolone or DHEA retained in Fisher microcentrifuge tubes after 1 hour incubation in D-PBS at concentrations of 2, 20, and 200 nM for each steroid. Experiment was performed twice in duplicate and graph shows mean ± SD. For pregnenolone across different concentrations, p = 0.43 from one-way ANOVA. For DHEA across different concentrations, p = 0.31 from one-way ANOVA.