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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2019 Dec 26;198:105572. doi: 10.1016/j.jsbmb.2019.105572

Figure 6. More steroid adsorption occurs at lower temperatures.

Figure 6.

Fraction of pregnenolone or DHEA retained in Fisher microcentrifuge tubes after 1 hour incubation in D-PBS at 4°C, 22°C, and 37°C. Experiment was performed twice in triplicate and graph shows mean ± SD. For both pregnenolone and DHEA, retention at 4°C was greater than at 22°C or 37°C (p < 0.001 for all comparisons, Tukey’s multiple comparison test after one-way ANOVA).