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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2019 Dec 26;198:105572. doi: 10.1016/j.jsbmb.2019.105572

Figure 7. Cellular affinity for a steroid outcompetes steroid adsorption to plastic.

Figure 7.

Fraction of pregnenolone collected in media, cell pellet or simulated pellet collection, and empty tube for one hour incubations of serum-free media with pregnenolone in Fisher microcentrifuge tubes with and without LNCaP prostate cancer cells (A) or HMC-1–8 breast cancer cells (B). Experiment was performed twice in triplicate (A) or three times in triplicate (B) and graphs shows mean ± SD. Retention in empty tubes was greater without cells than with cells for both cell lines (t-tests, p < 0.001).