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. 2020 Feb 24;71(10):3080–3093. doi: 10.1093/jxb/eraa099

Fig. 4.

Fig. 4.

Effect of PpMYB8 RNAi-mediated silencing in the regulation of Phe and phenylpropanoid biosynthesis and lignin deposition. (A) Average expression level of the nine ADT genes from P. pinaster in the RNAi-PpMYB8 lines, expressed as a percentage relative to the RNAi-EV control. (B) Equivalent analysis to (A) for some key genes involved in Phe biosynthesis and the first steps of the biosynthesis of phenylpropanoids. (C) Vascular anatomy in cross-sections of stems from RNAi-PpMYB8 transgenic plants (lines 11PP16 and 11PP17) and control plants (RNAi-EV). The first row shows phloroglucinol-HCl staining. The second and third rows show blue light autofluorescence. (D) Klason (black) and acid-soluble (gray) lignin determination in the stems from RNAi-EV control and RNAi-PpMYB8 plants (11PP16, 11PP17). Gene abbreviations in (A) and (B) are as described in the legend of Fig. 2. Error bars represent the SD; asterisks indicate significant differences by t-test (α=0.01; n=3) between transgenic and control RNAi-EV lines.