Fig. 3.

Map-based cloning and genetic complementation of the rice Osfc24 mutant. (A) The mutant locus was mapped between molecular markers C2 and C6 in a 214-kb region. The candidate locus is LOC_Os08g28730 (FC24). A nonsense mutation (E335Stop) is located in the 9th exon of FC24. (B) Schematic diagram of the pFC24F vector for the genetic complementation of the Osfc24 mutant. (C) Phenotypes of 60-d-old wild-type (WT), Osfc24, and pFC24F-complemented plants. (D) Specific amplification of the FC24 coding sequence in the genome DNA of WT, Osfc24, and pFC24F plants. (E) Co-segregation of F₂ mutant plants using the restriction enzyme Mse I, a specific restriction site that was newly generated by the mutation of the FC24 gene. The WT and the Osfc24 mutant were used as negative and positive controls, respectively. M, marker.