Table 4.

MID and average labeling abundance (%) per carbon of compounds specific to the cytosol or plastid

Compartmentalized metabolites	Mass isotopomers	Average labeling (%  13C abundance)
		[13C]GLC	[13C]Gln
AcCoAp	M+0	49.74±1.04	89.19±0.68
	M+1	10.64±0.48	4.59±0.36
	M+2	41.57±0.83	6.54±0.59
AcCoAc	M+0	59.30±1.14	48.07±0.85
	M+1	15.08±0.63	9.97±0.32
	M+2	25.62±1.84	41.96±1.28
H6Pp	M+0	9.99±0.87	92.16±0.71
	M+1	7.55±1.07	7.31±0.64
	M+2	49.44±6.16	0.52±0.33
	M+3	12.71±1.43	0.02±0.27
	M+4	6.71±2.92	0.00±0.25
	M+5	3.84±3.89	0.01±0.25
	M+6	9.97±0.61	0.00±0.25
G6Pc	M+0	7.96±1.16	92.37±1.14
	M+1	5.53±0.49	6.56±0.46
	M+2	43.30±0.61	0.81±0.97
	M+3	13.70±0.56	0.19±0.32
	M+4	9.75±0.56	0.05±0.27
	M+5	9.21±0.46	0.02±0.27
	M+6	10.92±0.35	0.01±0.26
F6Pc	M+0	7.56±1.21	92.66±0.46
	M+1	5.24±0.46	6.68±0.45
	M+2	42.79±0.59	0.44±0.30
	M+3	14.42±0.66	0.18±0.29
	M+4	9.92±0.55	0.03±0.27
	M+5	9.91±0.51	0.00±0.25
	M+6	10.44±0.47	0.00±0.26
GLCc	M+0	10.14±1.19	92.43±0.46
	M+1	3.08±1.01	6.86±0.43
	M+2	54.69±3.07	0.57±0.31
	M+3	8.42±1.12	0.11±0.33
	M+4	5.08±0.84	0.02±0.27
	M+5	4.57±0.93	0.01±0.26
	M+6	14.23±1.38	0.01±0.26

For the labeling information of plastidic and cytosolic acetyl-CoA, McLafferty fragments of palmitic (C16:0) and erucic acids (C22:1) were analyzed, respectively, by GC-MS (n=3). Starch and sucrose glucosyls were subject to LC-MS/MS to elucidate the labeling of plastidic and cytosolic hexoses, respectively. MID and the average labeling abundance (%) in each carbon were determined for the compounds listed from [¹³C]GLC and [¹³C]Gln experiments. The values are the average of three biological replicates ±SD. Cytosolic and plastidic compounds are denoted as ‘c’ and ‘p’, respectively.