Table 6.
MID and average labeling abundance (%) of organic acids
| Organic acids | Mass isotopomers | Average labeling (% 13C abundance) | |
|---|---|---|---|
| [13C]GLC | [13C]Gln | ||
| CIT | M+0 | 45.38±0.83 | 18.14±0.99 |
| M+1 | 18.90±0.54 | 6.58±0.48 | |
| M+2 | 18.56±0.35 | 6.17±0.52 | |
| M+3 | 8.37±0.44 | 11.32±0.52 | |
| M+4 | 5.37±0.39 | 16.30±1.03 | |
| M+5 | 2.73±0.32 | 33.76±1.20 | |
| M+6 | 0.76±0.28 | 8.38±1.00 | |
| ICIT | M+0 | 45.05±1.84 | 19.37±1.14 |
| M+1 | 19.28±0.83 | 6.97±0.52 | |
| M+2 | 18.53±1.12 | 6.13±0.82 | |
| M+3 | 8.39±0.71 | 11.60±0.95 | |
| M+4 | 5.35±0.59 | 15.78±1.20 | |
| M+5 | 2.72±0.35 | 32.04±1.44 | |
| M+6 | 0.75±0.29 | 8.74±0.81 | |
| MAL | M+0 | 54.61±1.07 | 35.82±0.45 |
| M+1 | 14.68±0.37 | 10.12±0.74 | |
| M+2 | 16.45±0.57 | 7.87±0.66 | |
| M+3 | 10.77±0.64 | 13.71±0.55 | |
| M+4 | 3.65±0.35 | 33.04±1.20 | |
| SUCC* | M+0 | 51.27±0.76 | 36.77±1.73 |
| M+1 | 21.36±1.51 | 6.76±0.69 | |
| M+2 | 19.93±0.82 | 12.72±1.40 | |
| M+3 | 3.60±0.55 | 4.01±0.93 | |
| M+4 | 3.85±0.40 | 39.75±2.25 | |
| FUM | M+0 | 59.20±1.36 | 37.64±1.13 |
| M+1 | 14.05±1.09 | 9.38±1.19 | |
| M+2 | 14.29±1.51 | 7.37±0.77 | |
| M+3 | 9.42±0.49 | 13.34±1.04 | |
| M+4 | 3.14±0.43 | 32.70±1.21 | |
Each mass isotopomer was detected and measured through LC-MS/MS in a negative ion mode. MID and the average labeling abundance (%) in each carbon were determined for organic acids from [13C]GLC and [13C]Gln experiments. The values listed are the average of three biological replicates ±SD. An asterisk (*) represents a metabolite whose MID values were corrected according to a dilution factor obtained from the 20% labeling experiment (Supplementary Table S6).