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. 2020 May 29;20:204. doi: 10.1186/s12935-020-01285-w

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Overexpression of LATS2-AS1-001 inhibits GC cell proliferation and colony formation. The expression pattern of LATS2-AS1-001 in GC cell lines is shown in a. Low expression of LATS2-AS1-001 was detected in BGC823 and SGC7901 cells compared with GES-1 cells. Ectopic expression of LATS2-AS1-001 in BGC823 and SGC7901 cell lines was analyzed by qRT-PCR (b, c). The viability of BGC823 (d) and SGC7901 (e) cells after stable transfection was detected by CCK-8. Ectopic LATS2-AS-001 expression decreased GC cell proliferation after 5 days (*P < 0.05). The colony-formation assay demonstrated that LATS2-AS1-001 overexpression significantly suppressed proliferation in BGC823 (f) and SGC7901 (g) cells. Histograms of average clone numbers are shown in h, i, *P < 0.05