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. 2020 May 29;20:204. doi: 10.1186/s12935-020-01285-w

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — LAST2-AS1-001 recruits EZH2 to upregulate LATS2, and promotes YAP1 phosphorylation. a RIP was performed to assess the interaction of LATS2-AS1-001 with EZH2. The enrichment of LATS2-AS1-001 with EZH2 was normalized to IgG. *P < 0.05. b The efficiency of EZH2 knockdown in BGC823 cells was assessed by qRT-PCR. c LATS2 mRNA levels were detected by qRT-PCR after EZH2 knockdown. LATS2 and YAP1 expression levels were measured by qRT-PCR and Western blot in BGC823 and SGC7901 cells overexpressing LAST2-AS1-001. The results showed that LATS-AS1-001 overexpression upregulated LATS2 (d, e, h), downregulated YAP1 (f–h) both at the mRNA and protein levels, and promoted YAP1 phosphorylation. In addition, the cell cycle related protein cyclin D1 was also downregulated after LATS-AS1-001 overexpression in GC cells (h)