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. 2020 May 29;21:206. doi: 10.1186/s12882-020-01861-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — BBG administration decreases P2X7-R immunoexpression in tubular cortex but not in medulla in UUO. a Graphical representation of P2X7 surface density in tubular cells in renal cortex. The graphic shows aligned dot plot and the mean ± SEM of 20 captured images of each animal kidney in the different experimental groups. * p < 0.05 vs UUO-BBG and both SHAM groups. # p < 0.05 vs UUO-V and both SHAM groups. b Representative immunostaining of the renal cortex from UUO-V group. The arrows indicate one immunostained interstitial cell. c UUO-BBG group. d Graphical representation of P2X7 surface density in tubular cells in renal medulla. The graphic shows aligned dot plot and the mean ± SEM of 20 captured images of each animal kidney in the different experimental groups. * p < 0.05 vs both SHAM groups, but not between UUO groups. Representative immunostaining of the renal medulla from (e) UUO-V group and (f) UUO-BBG group. All pictures of the sham control groups are shown in the supplementary material. Bar = 50 μm in all figures.