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. 2020 May 29;20:202. doi: 10.1186/s12935-020-01287-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Circ-CSPP1 promoted proliferation and inhibits apoptosis of NSCLC cells. a The schematic illustration of circ-CSPP1 expression vector and shRNAs. b The transfection efficiency of overexpression of circ-CSPP1 (named as circ-CSPP1) and sh-circ-CSPP1 in Hep3B and SK-HEP-1 cells was analyzed by qRT-PCR. c The effects of overexpression or knockdown of circ-CSPP1 on the mRNA expression of linear CSPP1. d CCK-8 assay was used to determine cell proliferation. e The colony formation assay was performed. (F) The cell apoptosis was determined by flow cytometry. g The migratory ability of HCC cells was decreased by wound healing assay. (H) The transwell assay showed the number of invasive cells. *p < 0.05; **p < 0.01 and ***p < 0.001