Table-I.
Genotype polymorphism | Primers | PCR conditions |
---|---|---|
IL-1B-511C/T | F 5′-GC CT GA AC CC TG CA TA CC GT-3′ R 5′-GG AA TC TT CC CA CT TA CA GA TGG-3′ |
Denaturation of DNA for 10 minutes at 95°C, 40 cycles for 1 minute at 95°C, annealing at 55°C extension at 72°C and final extension for 5 minute at 72°C. |
IL-1B-31T/C | F 5′-AG AA GC TT CC ACCAATAC TC-3′ R 5′-AC TAAC TT TA GG GT GT CAG-3′ |
10 min denaturation at 94°C, 36 cycles for 2 minute at 94°C, annealing at 54°C, extension at 72°C and final extension at 72°C for 5 min. |