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. 2020 May-Jun;36(4):825–830. doi: 10.12669/pjms.36.4.1883

Table-I.

PCR primers and conditions.

Genotype polymorphism Primers PCR conditions
IL-1B-511C/T F 5′-GC CT GA AC CC TG CA TA CC GT-3′
R 5′-GG AA TC TT CC CA CT TA CA GA TGG-3′
Denaturation of DNA for 10 minutes at 95°C, 40 cycles for 1 minute at 95°C, annealing at 55°C extension at 72°C and final extension for 5 minute at 72°C.
IL-1B-31T/C F 5′-AG AA GC TT CC ACCAATAC TC-3′
R 5′-AC TAAC TT TA GG GT GT CAG-3′
10 min denaturation at 94°C, 36 cycles for 2 minute at 94°C, annealing at 54°C, extension at 72°C and final extension at 72°C for 5 min.