Fig. 1.

(A) Aβ42 monomer concentration over time as monitored by the NMR signal intensity of methyl protons for reactions starting from 13.7 µM Aβ42 monomer at 37 °C at different pH values in 20 mM sodium phosphate buffer with 200 μM EDTA, 0.02% NaN₃, and 10% (vol/vol) D₂O. Representative data are shown, and repeats of the same conditions are shown in SI Appendix, Fig. S1. (B–D) ThT fluorescence intensity and light scattering from three independent experiments at 37 °C starting from 13.7 µM Aβ42 monomer at pH 6.8. Signals have been normalized to the approximate plateau values around 6 to 8 h.