Fig. 2.

Theoretical and experimental analysis of collagen mechanical properties. (A) Schematic illustrating the effect of shear stress on fiber alignment during rheology testing. A fraction of fibers aligns (red) with the first principal stretch direction leading to stiffening under loading. The fibers that do not align (black) remain randomly oriented and isotropic. (B) Representative stress–strain curves of cell-free cold- and warm-cast scaffolds as obtained by experimental analysis or computational simulation. (C) Average of shear moduli of cold- and warm-cast scaffolds obtained via rheological measurements and computational modeling. *P < 0.05 between conditions.