Fig. 2.
Phage U136B relies on LPS. (A) An EOP screen of phage U136B on LPS synthesis gene knockouts reveals genes important to phage replication: rfaC, rfaD, rfaE, and rfaP. A “bd” at the lower, dashed line indicates that the EOP was below the limit of detection (∼10−7). (B) Genetic complementation with plasmids containing respective rfa genes fully restores plaquing ability by phage U136B on the knockouts. Error bars = 95% CIs. (C) Schematic of genes involved in LPS synthesis, showing that the genes required by U136B affect the deep region of core polysaccharide (regions highlighted in red, blue, and yellow). Modified with permission from refs. 35 and 36, with permission from American Society for Microbiology, with additional data from refs. 37 and 38.