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. Author manuscript; available in PMC: 2021 Apr 17.
Published in final edited form as: ACS Synth Biol. 2020 Apr 7;9(4):893–901. doi: 10.1021/acssynbio.9b00516

Figure 3:

Figure 3:

Spatiotemporal controlled generation of positive membrane curvature by opto-FBAR. A) COS7 cells expressing the opto-FBAR system were exposed to blue light for 5 minutes, which induced significant membrane tubules. Light-induced positive membrane curvature is fully reversed after 30 min in the dark. Full field images available in Supplementary Figure S5. Scale bar = 1 μm. B) A subcellular region (areas to the left of the blue line) of COS7 cells expressing opto-FBAR system were exposed to blue light for 5 minutes. Membrane tubules are induced in the region exposed to blue light. Full field images available in Supplementary Figure S6. Scale bar = 5 μm. C) Green channel (iLID-EGFP-CAAX) were analyzed with TubuleTracer to quantify the tubulation extent over time. The results are shown under the raw images. Scale bar = 10 μm. D) The plot of tubulation area vs time from the cell shown in C. Tubulation reaches a maximum around 2 min.