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. Author manuscript; available in PMC: 2021 Apr 17.
Published in final edited form as: ACS Synth Biol. 2020 Apr 7;9(4):893–901. doi: 10.1021/acssynbio.9b00516

Figure 5:

Figure 5:

A) Plots of filopodia number and total filopodia length from FiloQuant analysis of cell shown in Figure 4D. B) Zoomed images of U2OS cells expressing the opto-IBAR system and a LifeAct-FusionRed F-actin marker. Cells were activated with blue light for 15 minutes as described in Methods. Most filopodia contain F-actin, but some long filopodia are devoid of F-actin (arrows). Scale bars = 5 μm. Note: Green channel images were bleach corrected using the histogram matching method in ImageJ. Full field images available in Supplementary Figure S10.