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. 2020 May 13;117(21):11636–11647. doi: 10.1073/pnas.1920570117

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Fig. 4. — Differential binding of KIR3DL1 to HLA-B*57:01 and HLA-B*57:03-presented peptides. SPR measurements of soluble KIR3DL1*001 binding to HLA-B*57:01 (A) or HLA-B*57:03 (B) with the peptides LF9 (i), LY9 (ii) or AW10 (iii). Equilibrium analysis of duplicate concentration series (108.0 to 1.69 μM) from which affinity (K_D) was calculated (Inset value ± SE). Data are shown as mean ± range of duplicate injections (n.b., no binding). Inset graphs show reference subtracted sensograms of each injection. Data are representative of two independent experiments.