Additional ablation of FoxO1 in adipocytes does not restore adipokine levels and adipose tissue mass in A-PDK1KO mice. (A) Plasma cholesterol and triglyceride as well as (B) adiponectin and leptin concentrations in control, A-PDK1KO, or A-PDK1/FoxO1DKO mice (n = 7 to 10). (C) Weight of epididymal and s.c. adipose tissue in control, A-PDK1KO, or A-PDK1/FoxO1DKO mice (n = 5 or 6). (D) RT and real-time PCR analysis of the expression of genes related to inflammation in epididymal adipose tissue of control, A-PDK1KO, or A-PDK1/FoxO1DKO mice (n = 5 or 6). All data are means ± SEM; *P < 0.05, **P < 0.01 (Student’s t test).