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Antiviral effect of gemcitabine against RRV rotavirus strain and clinical isolates. qRT-PCR analysis of rotavirus RNA upon treatment of gemcitabine (GCT) at 1 μM for 48 h in laboratory rotavirus strain RRV (A), clinically isolated rotavirus strain 026k (B), 1758k (C), 1934 (D), 664k (E), and 2011k (F). Data were normalized to housekeeping gene GAPDH and are presented relative to the control (CTR) (set as 1). Data represent means ± SEM; n = 6; *P < 0.05; **P < 0.01.
