Table 3. Reported human T-cell epitopes in the H5N1 highly conserved sequences.
| Protein | H5N1 highly conserved sequence a | Immunogenic T-cell epitopeb | |||
|---|---|---|---|---|---|
| EpitopeIDc | Sequenced | Tsubset | HLA Allele | ||
| M1 | 175 HENRMVLAS 183 | 65389 | TNPLIRHENRMVLASTTAKA | CD4+ | HLA-DRB1*01:03, HLA-DRB1*15:01, HLA-DRB5*01:01, HLA-DRB1*07:01, HLA-DRB1*11:01, HLA-DRB1*15:02, HLA-DRB1*03:01 |
| 148697 | NPLIRHENRMVLAST | CD4+ | HLA-DR | ||
| 79948 | NPLIRHENRMVLAS | CD4+ | HLA DRA*01:01/DRB1*07:01 | ||
| 113695 | PLIRHENRMVLAST | CD4+ | HLA-DRB1*03:01 | ||
| 97403 | IRHENRMVLASTTAKAM | CD4+ | – | ||
| 97395 | IKHENRMVLASTTAKAM | CD4+ | – | ||
| NP | 68 LSAFDERRN 76 | 54595 | RLIQNSLTIERMVLSAFDERRNK | – | – |
| 97689 | TIEKMVLSAFDERRNKYL | – | – | ||
| 54955 | RMVLSAFDERRNRYLEEHPS | CD4+ | HLA-DRB1*01:01 | ||
| 164364 | RMVLSAFDERRNKYLEEH | CD4+ | – | ||
| 137 MIWHSNLND 145 | 41793 | MIWHSNLNDATYQRTRALVR | CD4+ | HLA-DRB1*07:01, HLA-DRB5*01:01 | |
| 218 AYERMCNILKGK 229 | 18366 | FWRGENGRKTRSAYERMCNILKGK | CD4+ | HLA-DR1, HLA-DR2 | |
| 97621 | RRTRIAYERMCNILKGKF | CD4+ | – | ||
| 97607 | RKTRSAYERMCNILKGKF | CD4+ | – | ||
| 145824 | IAYERMCNILKGKFQTAA | CD8+ | HLA-B*15:01 | ||
| 181251 | YERMCNILKG | CD8+ | HLA-B44, HLA-B*18:01 | ||
| 271 AHKSCLPACVYG 282 | 4255 | ARSALILRGSVAHKSCLPACVYGP | – | – | |
| 27284 | ILRGSVAHKSCLPACVYGLA | CD4+ | HLA-DRB1*07:01 | ||
| 33285 | KSCLPACVY | CD8+ | HLA-A*01:01, HLA-A*30:02 | ||
| PA | 583 RCLLQSLQQ 591 | 129181 | KWGMEMRRCLLQSLQQI | – | – |
| PB1 | 26 GDPPYSHGTGTGY 38 | 97682 | TFPYTGDPPYSHGTGTGY | CD8+ | – |
| 75755 | YSHGTGTGY | CD8+ | HLA-A*01:01, HLA-A*26:01HLA-A*30:02, HLA-A*29:02 | ||
| 337 LSIAPIMFS 345 | 236661 | YITRNQPEWFRNVLSIAPIMFS NKMARLGKGYMFE | – | – | |
| 483463 | QPEWFRNVLSIAPIMFSNK | – | HLA-DR | ||
| 148616 | FRNVLSIAPIMFSNKM | CD4+ | HLA-DR | ||
| 401 ASLSPGMMMGMFNMLSt 417 | 42143 | MMMGMFNML | – | HLA-A2 | |
| 500 GFVANFSMELPS 511 | 97627 | RYGFVANFSMELPSFGV | CD8+ | – | |
| 97314 | FVANFSMEL | – | HLA-A2, HLA-A*02:01 | ||
| 537 NDLGPATaqMAlqlfiK 553 | 21574 | GPATAQMAL | CD8+ | HLA-B7 | |
| 555 YRYTYRCHRGD 565 | 1960900 | DYRYTYRCHRGDTQIQT | CD4+ | HLA-DRB1*03:01 | |
Notes.
a
Amino acids are numbered according to the sequence alignment of the respective protein. Positions that do not satisfy the concatenating criteria are indicated as small letter case. Sequences that matched with Heiny et al. (2007) data are underlined.
b
Reported T-cell epitopes from the Immune Epitope Database (IEDB). Dashes, not determined.
c
Epitope ID based on IEDB (http://www.iedb.org/).
d
Immunogenic sequences in IEDB that matched the highly conserved sequences are bold in red.